Journal: Surface and Coatings Technology

Article Title: Comparison of HIPIMS sputtered Ag- and Cu-surfaces leading to accelerated bacterial inactivation in the dark

doi: 10.1016/j.surfcoat.2014.02.029

Figure Lengend Snippet: Fig. 4. E. coli inactivation as a function of time for Ag-polyester sputtered at 5 A for different times in the dark.

Article Snippet: The samples of Escherichia coli (E. coli K12) was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ) ATCC23716, Braunschweig, Germany, to test the antibacterial activity of the Ag and Cu sputtered samples.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Inhibition of ANGPTL8 protects against diabetes-associated cognitive dysfunction by reducing synaptic loss via the PirB signaling pathway

doi: 10.1186/s12974-024-03183-8

Figure Lengend Snippet: ANGPTL8 levels were increased in the serum and CSF of patients and mice with diabetes-associated cognitive dysfunction. A Serum ANGPTL8 levels in the indicated groups (n = 10 per group). B ANGPTL8 levels in the CSF of the indicated groups (n = 10 per group). C Schematic depicting the novel object recognition task (the green square represents the familiar object, and the purple square represents the novel object). F, familiar object; N, novel object. D Representative traces of paths by the indicated mice to explore novel objects. E , F Recognition index of the indicated mice, calculated as the difference in the amount of time spent exploring the novel and familiar objects (left) and in the frequency of novel object explorations and familiar object explorations (right) (n = 4–7 mice per group). G Representative traces of paths to the target hole (red) by the indicated mice on the day of testing in the Barnes maze. H , I Barnes maze acquisition and probe trials for the indicated mice, shown by average errors. J Serum ANGPTL8 levels in the indicated groups. K CSF ANGPTL8 levels in the indicated groups. L Angptl8 mRNA expression in the hippocampus in the indicated groups. M Immunofluorescence analysis of ANGPTL8 protein expression in the hippocampal CA1 region of the indicated mice. N Immunofluorescence staining of IBA-1 (red) and CD11b (green) in the hippocampal CA1 region of the indicated mice. O mRNA expression of proinflammatory cytokines ( Il-1β , Il-6 , and Tnf-α ) and axonal growth and synaptic plasticity markers ( Gap-43 , Snap-25 , Nfl , and Syp ) in the hippocampus of the indicated groups. The data are shown as the mean ± s.e.m. and were statistically analyzed by two-way ANOVA with Bonferroni’s test ( A , B ) and two-tailed Student’s t test ( C – O ). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. NS: nonsignificant. CSF: cerebrospinal fluid; HC: healthy control; T2DM: type 2 diabetes mellitus

Article Snippet: The antibodies included FITC-CD11b (BioLegend, CA, USA, #101206; 1 μg per 10 6 cells), APC-CD206 (BioLegend, CA, USA, #141707; 2 μg per 10 6 cells), and PE-Iba-1 (Cell Signaling Technology, USA, #10513; 1 μg per 10 6 cells).

Techniques: Expressing, Immunofluorescence, Staining, Two Tailed Test, Control

Journal: Journal of Neuroinflammation

Article Title: Inhibition of ANGPTL8 protects against diabetes-associated cognitive dysfunction by reducing synaptic loss via the PirB signaling pathway

doi: 10.1186/s12974-024-03183-8

Figure Lengend Snippet: Depletion of Angptl8 in the central nervous system (CNS) protected against diabetes-associated cognitive dysfunction. A Strategy used to generate Angptl8 Nestin CKO mice. B ANGPTL8 levels in CSF in the indicated groups (n = 5 mice per group). C Western blotting analysis of ANGPTL8 in the brain tissue of the indicated mice. D Immunofluorescence staining showing Angptl8 knockout efficiency in the hippocampus of HFD-fed Angptl8 conditional knockout (CKO) mice. E Nissl staining images showing Nissl bodies in the hippocampus of male Loxp/loxp and Angptl8 Nestin CKO mice (left). Quantitative analysis of the number of neurons (right) (n = 5 per group). F Double immunostaining of NeuN (green) and IBA-1 (red) in the hippocampus of male Loxp/loxp and Angptl8 Nestin CKO mice (left). Quantitative analysis of the number of NeuN + and IBA-1 + cells (right) (n = 5 per group). G Representative traces of paths by the indicated mice to explore a novel object (the green square represents the familiar object, and the purple square represents the novel object). F, familiar object; N, novel object. H Recognition index of the indicated mice (n = 5 mice per group), calculated as the difference in the amount of time spent exploring the novel and familiar objects (left) and in the frequency of novel object explorations and familiar object explorations (right). I Traces of paths to the target hole (red) in Barnes maze probe trials for the indicated mice. J Barnes maze acquisition and probe trials for the indicated groups (n = 5 mice per group). * P < 0.05, ** P < 0.01, **** P < 0.0001; + P < 0.05: Loxp/loxp + HFD group vs. Angptl8 Nestin CKO + HFD group; ## P < 0.01: Loxp/loxp + ND group vs. Loxp/loxp + HFD group; Δ P < 0.05: Angptl8 Nestin CKO + ND group vs. Angptl8 Nestin CKO + HFD group. NS, nonsignificant. The data are shown as the mean ± s.e.m. and were statistically analyzed by two-way ANOVA with Bonferroni’s test. CSF: cerebrospinal fluid

Article Snippet: The antibodies included FITC-CD11b (BioLegend, CA, USA, #101206; 1 μg per 10 6 cells), APC-CD206 (BioLegend, CA, USA, #141707; 2 μg per 10 6 cells), and PE-Iba-1 (Cell Signaling Technology, USA, #10513; 1 μg per 10 6 cells).

Techniques: Western Blot, Immunofluorescence, Staining, Knock-Out, Double Immunostaining

Journal: Journal of Neuroinflammation

Article Title: Inhibition of ANGPTL8 protects against diabetes-associated cognitive dysfunction by reducing synaptic loss via the PirB signaling pathway

doi: 10.1186/s12974-024-03183-8

Figure Lengend Snippet: Depletion of Angptl8 in the CNS protected against synaptic damage and alleviated neuroinflammation. A Body weight of the indicated mice. B , C GTT and area-under-the-curve (AUC) values of the indicated mice. D , E ITT and AUC values of the indicated mice. F Hippocampal TG levels of the indicated mice. G Immunofluorescence staining of IBA-1 (red) and CD11b (green) in the CA1 region of the hippocampus in the indicated mice. H Immunofluorescence staining of CD16/32 (red) and CD206 (green) in the hippocampal CA1 region in the indicated mice. I Representative images of synaptic ultrastructure (red arrow) in the hippocampus. J Quantification of the number of synapses in the indicated mice. K mRNA expression of proinflammatory cytokines ( Il-1β , Il-6 , and Tnf-α ) and axon growth and synaptic plasticity markers ( Gap-43 , Snap-25 , Nfl , and Syp ) in the hippocampus of the indicated groups (n = 5 mice per group). * P < 0.05, ** P < 0.01, *** P < 0.001; # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001: Loxp/loxp + ND group vs Loxp/loxp + HFD group; ΔP < 0.05, ΔΔ P < 0.01, ΔΔΔ P < 0.001, ΔΔΔΔ P < 0.0001: Angptl8 Nestin CKO + ND group vs Angptl8 Nestin CKO + HFD group. NS: nonsignificant. The data are shown as the mean ± s.e.m. and were statistically analyzed by two-way ANOVA with Bonferroni’s test

Article Snippet: The antibodies included FITC-CD11b (BioLegend, CA, USA, #101206; 1 μg per 10 6 cells), APC-CD206 (BioLegend, CA, USA, #141707; 2 μg per 10 6 cells), and PE-Iba-1 (Cell Signaling Technology, USA, #10513; 1 μg per 10 6 cells).

Techniques: Immunofluorescence, Staining, Expressing

Journal: Journal of Neuroinflammation

Article Title: Inhibition of ANGPTL8 protects against diabetes-associated cognitive dysfunction by reducing synaptic loss via the PirB signaling pathway

doi: 10.1186/s12974-024-03183-8

Figure Lengend Snippet: PirB −/− mice were resistant to ANGPTL8-induced neuroinflammation and synaptic injury. A Experimental scheme. B Representative traces of paths by the indicated mice to explore a novel object (the green square represents the familiar object, and the purple square represents the novel object). F, familiar object; N, novel object. C Recognition index of the indicated mice (n = 5 mice per group), calculated as the difference in the amount of time spent exploring the novel and familiar objects (left) and in the frequency of novel object explorations and familiar object explorations (right). D Traces of paths to the target hole (red) in Barnes maze probe trials for the indicated mice. E Barnes maze acquisition and probe trials for the indicated groups (n = 5 mice per group). F Immunofluorescence staining of IBA-1 (red) and CD11b (green) in the hippocampal CA1 region of the indicated mice. G Representative images of synaptic ultrastructure (red arrow) in the hippocampus of the indicated mice. H Quantification of the number of synapses in the hippocampus of the indicated mice. I mRNA expression of proinflammatory cytokines ( Il-1β , Il-6 , and Tnf-α ) and axonal growth and synaptic plasticity markers ( Gap-43 , Snap-25 , Nfl , and Syp ) in the hippocampus of the indicated groups. * P < 0.05, ** P < 0.01, *** P < 0.001; + P < 0.05, ++ P < 0.01: WT + rA8 group vs. PirB −/− + rA8 group; # P < 0.05, ## P < 0.01, ### P < 0.001: WT + Scrambled group vs. WT + rA8 group. NS, nonsignificant. The data are shown as the mean ± s.e.m. (n = 5 mice per group) and were statistically analyzed by two-way ANOVA with Bonferroni’s test. rA8, flag-targeted recombinant ANGPTL8 protein

Article Snippet: The antibodies included FITC-CD11b (BioLegend, CA, USA, #101206; 1 μg per 10 6 cells), APC-CD206 (BioLegend, CA, USA, #141707; 2 μg per 10 6 cells), and PE-Iba-1 (Cell Signaling Technology, USA, #10513; 1 μg per 10 6 cells).

Techniques: Immunofluorescence, Staining, Expressing, Recombinant

Journal: Oxidative medicine and cellular longevity

Article Title: Spermidine Prevents Heart Injury in Neonatal Rats Exposed to Intrauterine Hypoxia by Inhibiting Oxidative Stress and Mitochondrial Fragmentation.

doi: 10.1155/2019/5406468

Figure Lengend Snippet: Figure 3: Observation of myocardial morphological structure, cell proliferation, apoptosis, and fibrosis in offspring rats. (a) Representative left ventricle sections stained by HE in the normal (control), hypoxia (Hpx), SPD-treated (Hpx-Spd), and SPD+polyamine synthesis inhibitor- (Hpx-Spd-DMFO-) treated groups. (b) Representative immunohistochemical staining for protein expression and localization of MCM2-positive cardiomyocytes. (c) Brown-stained nuclei indicate TUNEL-positive cells. (d) Evaluation of the percentage of binucleated cardiomyocytes (n = 6). (e) Evaluation of the percentage of MCM2-positive cells (n = 10). (f) The percentage of TUNEL-positive nuclei in different groups (n = 8). (g) BAX and BCL2 protein expression detected by western blotting. (h) Quantification of the BAX and BCL2 protein level ratio (n = 4). (i) Representative Masson’s trichrome staining in ventricle sections in each group. (j) Evaluation of interstitial fibrotic areas in ventricle sections in each group (n = 8). Data are shown as the mean ± SEM. ∗P < 0 05 versus control, #P < 0 05 versus the Hpx group, and △P < 0 05 versus the Hpx-Spd group.

Article Snippet: The tissues were incubated with 1% bovine serum albumin for 20 min and then incubated overnight with primary antibody against MCM2 (1 : 150, 105-1-AP, Proteintech, Wuhan, China).

Techniques: Staining, Control, Immunohistochemical staining, Expressing, TUNEL Assay, Western Blot

Journal: Scientific Reports

Article Title: Stimulation of carbon nanomaterials on syntrophic oxidation of butyrate in sediment enrichments and a defined coculture

doi: 10.1038/s41598-018-30745-7

Figure Lengend Snippet: Effects of the addition of CNTs and rGO on the CH 4 production ( a ) and acetate production ( b ), butyrate degradation ( c ) and butyrate degradation rate ( d ) in the defined coculture of S . wolfei with M . mariplaudis . Results are the mean and standard deviation for triplicate incubations. Different letters indicate significant differences (Duncan’s test, P < 0.05).

Article Snippet: Syntrophomonas wolfei (DSM102351) and Methanococcus maripaludis (DSM14266) were purchased from German culture collection DSMZ (Braunschweig, Germany).

Techniques: Standard Deviation

Journal: Scientific Reports

Article Title: Stimulation of carbon nanomaterials on syntrophic oxidation of butyrate in sediment enrichments and a defined coculture

doi: 10.1038/s41598-018-30745-7

Figure Lengend Snippet: FISH ( a , b ) and SEM ( c , d ) images of the defined coculture of S . wolfei with M . mariplaudis in the presence of CNTs ( a , c ) and rGO ( b , d ). The concentrations of CNTs and rGO are 2 g L −1 and 0.1 g L −1 , respectively. For the FISH images, red color indicates bacterial cell and green color indicates archaeal cell.

Article Snippet: Syntrophomonas wolfei (DSM102351) and Methanococcus maripaludis (DSM14266) were purchased from German culture collection DSMZ (Braunschweig, Germany).

Techniques:

Journal: ISME Communications

Article Title: Particle-associated bacteria differentially influence the aggregation of the marine diatom Minutocellus polymorphus

doi: 10.1038/s43705-022-00146-z

Figure Lengend Snippet: Outline of the experiments performed in this study.

Article Snippet: Stock cultures of Vibrio thalassae (DSM102810, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), Pseudoalteromonas carrageenovora (DSM6820, DSMZ), and Marinobacter adhaerens HP15 [ ] were maintained on Marine Agar (BD Difco 2216, Becton Dickinson, NJ; ZoBell, 1941) plates at 23 ± 1 °C.

Techniques: Bacteria, Sedimentation

Journal: ISME Communications

Article Title: Particle-associated bacteria differentially influence the aggregation of the marine diatom Minutocellus polymorphus

doi: 10.1038/s43705-022-00146-z

Figure Lengend Snippet: Diatom cell concentration in growth experiment 2 with axenic Minutocellus polymorphus ( a ) and diatom and bacteria cell concentration in co-culture treatments with M. polymorphus and the addition of Pseudoalteromonas carrageenovora ( b ) or Vibrio thalassae ( c ), as well as total volume of suspended micro-aggregates ( d ) and transparent exopolymeric particle (TEP) concentrations ( e ) in all treatments. Symbols and error bars denote the means and standard deviations of triplicate treatments.

Article Snippet: Stock cultures of Vibrio thalassae (DSM102810, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), Pseudoalteromonas carrageenovora (DSM6820, DSMZ), and Marinobacter adhaerens HP15 [ ] were maintained on Marine Agar (BD Difco 2216, Becton Dickinson, NJ; ZoBell, 1941) plates at 23 ± 1 °C.

Techniques: Concentration Assay, Bacteria, Co-Culture Assay

Journal: ISME Communications

Article Title: Particle-associated bacteria differentially influence the aggregation of the marine diatom Minutocellus polymorphus

doi: 10.1038/s43705-022-00146-z

Figure Lengend Snippet: Epifluorescence microscopy images of axenic Minutocellus polymorphus ( a , b ) and co-cultures of M. polymorphus and Marinobacter adhaerens HP15 ( c , d ), Vibrio thalassae ( e , f ), and Pseudoalteromonas carrageenovora ( g , h ) under blue ( a , c , e , g ) and UV ( b , d , f , h ) excitation wavelengths. Images were taken during the exponential phases of growth. Red: chlorophyll- a autofluorescence, blue: DAPI-bound nucleic acids. White arrows: diatom-attached bacteria; blue arrows: bacterial aggregates. Amorphous material is considered TEP.

Article Snippet: Stock cultures of Vibrio thalassae (DSM102810, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), Pseudoalteromonas carrageenovora (DSM6820, DSMZ), and Marinobacter adhaerens HP15 [ ] were maintained on Marine Agar (BD Difco 2216, Becton Dickinson, NJ; ZoBell, 1941) plates at 23 ± 1 °C.

Techniques: Epifluorescence Microscopy, Bacteria

Journal: ISME Communications

Article Title: Particle-associated bacteria differentially influence the aggregation of the marine diatom Minutocellus polymorphus

doi: 10.1038/s43705-022-00146-z

Figure Lengend Snippet: Transparent exopolymeric particle (TEP) production, TEP concentration normalized to diatom cell abundance, and volume of suspended micro-aggregates during the exponential, stationary, and decline growth phases of Minutocellus polymorphus in axenic cultures and in co-cultures with Marinobacter adhaerens HP15, Vibrio thalassae , or Pseudoalteromonas carrageenovora .

Article Snippet: Stock cultures of Vibrio thalassae (DSM102810, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), Pseudoalteromonas carrageenovora (DSM6820, DSMZ), and Marinobacter adhaerens HP15 [ ] were maintained on Marine Agar (BD Difco 2216, Becton Dickinson, NJ; ZoBell, 1941) plates at 23 ± 1 °C.

Techniques: Concentration Assay

Journal: ISME Communications

Article Title: Particle-associated bacteria differentially influence the aggregation of the marine diatom Minutocellus polymorphus

doi: 10.1038/s43705-022-00146-z

Figure Lengend Snippet: Abundance of aggregates formed in roller tank experiments inoculated to lower total cell abundances (10 3 cells mL −1 ).

Article Snippet: Stock cultures of Vibrio thalassae (DSM102810, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), Pseudoalteromonas carrageenovora (DSM6820, DSMZ), and Marinobacter adhaerens HP15 [ ] were maintained on Marine Agar (BD Difco 2216, Becton Dickinson, NJ; ZoBell, 1941) plates at 23 ± 1 °C.

Techniques: Sedimentation

Journal: ISME Communications

Article Title: Particle-associated bacteria differentially influence the aggregation of the marine diatom Minutocellus polymorphus

doi: 10.1038/s43705-022-00146-z

Figure Lengend Snippet: Number of visible sinking aggregates formed in roller tanks with axenic Minutocellus polymorphus , and M. polymorphus with the addition of 2.5 µm silica beads , Marinobacter adhaerens HP15, Vibrio thalassae , or Pseudoalteromonas carrageenovora in the low cell abundance experiments ( a ca. 10 cells mL −1 ; Table ) and of axenic M. polymorphus and M. polymorphus with the addition of M. adhaerens in the high cell abundance experiments ( b 10 5 cells mL −1 ; Table ). Lowercase letters indicate significant differences between treatments.

Article Snippet: Stock cultures of Vibrio thalassae (DSM102810, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH), Pseudoalteromonas carrageenovora (DSM6820, DSMZ), and Marinobacter adhaerens HP15 [ ] were maintained on Marine Agar (BD Difco 2216, Becton Dickinson, NJ; ZoBell, 1941) plates at 23 ± 1 °C.

Techniques: